Articles on Detection of
Gastrointestinal Infections through DNA Extraction
Directly from Stool Specimens
Click on the title to view the article
Click on the Pubmed ID to view the abstract on Pubmed
Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, Yao JD, Wengenack NL, Rosenblatt JE, Cockerill FR 3rd, Smith TF. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev. 2006 Jan;19(1):165-256. Review. Erratum in: Clin Microbiol Rev. 2006 Jul;19(3):595. PMID: 16418529
Ng CT, Gilchrist CA, Lane A, Roy S, Haque R, Houpt ER. Multiplex real-time PCR assay using Scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples. J Clin Microbiol. 2005 Mar;43(3):1256-60. PMID: 15750093
Stensvold R, Brillowska-Dabrowska A, Nielsen HV, Arendrup MC. Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction. J Parasitol. 2006 Oct;92(5):1081-7. PMID: 17152954
Blessmann J, Buss H, Nu PA, Dinh BT, Ngo QT, Van AL, Alla MD, Jackson TF, Ravdin JI, Tannich E. Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J Clin Microbiol. 2002 Dec;40(12):4413-7. PMID: 12454128
Parr JB, Sevilleja JE, Samie A, Alcantara C, Stroup SE, Kohli A, Fayer R, Lima AA, Houpt ER, Guerrant RL. Detection and quantification of Cryptosporidium in HCT-8 cells and human fecal specimens using real-time polymerase chain reaction.Am J Trop Med Hyg. 2007 May;76(5):938-42. Erratum in: Am J Trop Med Hyg. 2007 Jul;77(1):200. PMID: 17488919
Haque R, Roy S, Siddique A, Mondal U, Rahman SM, Mondal D, Houpt E, Petri WA Jr. Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp.Am J Trop Med Hyg. 2007 Apr;76(4):713-7. PMID: 17426176
Lier T, Simonsen GS, Haaheim H, Hjelmevoll SO,
Vennervald BJ, Johansen MV.
Novel real-time PCr for detection of Schistosoma japonicum in stool. Southeast
Asian J Trop Med Public Health. 2006 Mar;37(2):257-64. PMID:
17124983
Sunnotel O, Snelling WJ, Xiao L, Moule K, Moore JE, Millar BC, Dooley JS, Lowery
CJ.
Rapid and sensitive detection of single cryptosporidium oocysts from archived
glass slides. J Clin Microbiol. 2006 Sep;44(9):3285-91. PMID:
16954262
Summary of Detection Limits
|
Organism |
Author |
Year
|
Extraction and PCR Method |
Detection Limit |
Link to Paper |
|
Blastocystis |
Stensvold |
2006 |
QIAamp PCR |
32 cells/200mg |
|
|
Entamoeba histolytica |
Blessmann |
2002 |
RT-PCR FRET |
0.1 cell/gram |
|
| Cryptosporidium | Parr | 2007 | RT-PCR | 1000 oocysts/sample | Link |
|
Multiplex: Entamoeba histolytica, Giardia, Cryprosporidium |
Haque | 2007 | RT-PCR | ?? | Link |
| S. japonicum eggs | Lier | 2006 |
RT-PCR QIAamp |
1 egg/sample | Link |
| Enterocytozoon bieneusi and Encephalitozoon spp | Verweij | 2007 | RT-PCR | ?? | Link |
| Cryptosporidium (from slides) | Sunnotel | 2006 | RT-PCR | 1 oocyst | Link |
| Giardia | Ng | 2005 | RT-PCR |
20 cells/reaction and 200 cells/reaction |
Link |
Others
Article on reduced sensitivity of MagNA Pure LC System (Schuurman, 2005)